Azopirillum brasilense produces the auxin-like phenylacetic acid by using the key enzyme for indole-3-acetic acid biosynthesis

Article Abstract:

An antimicrobial compound is isolated from Azopirillum brasilense culture extracts by high-performance liquid chromatography and is further identified by gas chromatography-mass spectrometry as the auxin0like molecule, phenylacetic acid (PAA). The results of the PAA synthesis is found to be mediated by the indole-3-pyruvate decarboxylase, previously identified as key enzyme in indole-3-acetic acid (IAA) production in Azospirillum brasilense.

author: Somers, E., Srinivasan, M., Ptacek, D., Vanderleyden, J.
Auxin, Auxins, Gas chromatography, Phenylacetic acid

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Cloning and characterization of a novel [beta]-transaminase from Mesorhizobium sp. strain LUK: A new biocatalyst for the synthesis of enantiomerically pure [beta]-amino acids

Article Abstract:

The cloning of a novel [beta]-transaminase gene from Mesorhizobium sp. strain LUK showing a high activity for [beta]-aminocarboxylic acid is described. The results have shown that [beta]-transminase of Mesorhizobium sp. strain LUK is a promising biocatalyst for the preparation of chiral [beta]-aminocarboxylic acids.

author: Byung-Gee Kim, Hyungdon Yun, Dohyun Kyung, Juhan Kim, Minho Cha, Joo-Hyun Seo, Byung-Kwan Cho
Aminotransferases, Transaminases, Amino acids, Amino acid synthesis

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Novel method for detection of butanolides in Streptomyces coelicolor culture broth, using a His-tagged receptor (ScbR) and mass spectrometry

Article Abstract:

A successful model system for the identification of SCB1 from Streptomyces coelicolor is reported. Using S. coelicolor as a model system, SCB1 was detected from only 100 ml of the culture broth after solvent extraction.

author: Yung-Hun Yang, Hwang-Soo Joo, Kwangwon Lee, Kwang-Kyung Liou, Byung-Gee Kim, Hei-Chan Lee, Jae-Kyung Sohng
Genetic aspects, Streptomyces, Genetic research

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subjects list: Research, Mass spectrometry
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