Assessment of the genetic diversity among strains of Xanthomonas cynarae by randomly amplified polymorphic DNA analysis and development of specific characterized amplified regions for the rapid identification of X. cynarae

Article Abstract:

Researchers identified four markers in Xanthomonas cynarae by randomly amplified polymorphic DNA (RAPD) and created a PCR primer pair for each one. X. cynarae causes bacterial bract spot disease of artichokes and these primers could be used to detect X. cynarae in artichoke fields.

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Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-restriction fragment length polymorphism analysis of rRNA operons with special emphasis on P. syringae pv. tomato

Article Abstract:

PCR-restriction fragment length polymorphism (RFLP), with 14 restriction enzymes, reveals that 77 bacterial strains of Pseudomonas syringae and P. viridiflava fail to show any difference in rrs and rrl genes. The method also gives the nucleotide sequence of the internal transcribed spacer between the rRNA operons, rrs and rrl, for P. syringae pv. strain CFBP1392. RFLP patterns form three clusters, of which one contains all strains of P. syringae pathovar tomato and other pathovars.

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Comparison of randomly amplified polymorphic DNA with amplified fragment length polymorphism to assess genetic diversity and genetic relatedness within genospecies III of Pseudomonas syringae

Article Abstract:

Research was conducted to examine the genetic diversity and genetic relatedness of 23 strains belonging to the Pseudomonas syringae genospecies III. The methologies used were amplified fragment length polymorphic (AFLP) techniques and randomly amplified polymorphic DNA (RAPD). Experimental data revealed that the AFLP technique was more effective in the assessment of intrapathovar diversity than the RAPD method.

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