Genotypic and phenotypic diversity of phlD-containing Pseudomonas strains isolated from the rhizosphere of wheat

Article Abstract:

Research has been conducted on the fluorescent Pseudomonas spp. strains that produce 2,4-diacetylphloroglucinol in the rhizosphere. The isolation of these strains from wheat rhizosphere has been carried out.

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Assessment of genotypic diversity of antibiotic-producing Pseudomonas species in the rhizosphere by denaturing gradient gel electrophoresis

Article Abstract:

A simple and rapid method was developed to determine the presence and genotypic diversity of 2,4-diacetylphloroglucinol (DAPG)-producing Pseudomonas strains in rhizosphere samples. The results demonstrated that denaturing gradient gel electrophoresis (DGGE) analysis of the phlD gene allows identification of new genotypic groups of specific antibiotic-producing Pseudomonas with different abilities to colonize the rhizosphere of sugar of sugar beet seedlings.

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Conservation of the 2,4-diacetylphloroglucinol biosynthesis locus among fluorescent Pseudomonas strains from diverse geographic locations

Article Abstract:

The gene encoding the antibiotic, 2,4-diacetylphloroglucinol (PHL) is conserved in all the 45 fluorescent Pseudomonas strains that produce PHL. However, the strains show significant phenotypic and genotypic variation and originate from different geographical regions. There are two phenotypic groups. One group produces PHL, hydrogen cyanide (HCN) and pyoluteorin (PLT) while the other produces PHL and HCN. The strains that produce both PHL and PTL show genetic similarity while those that produce only PHL are genetically dissimilar.

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