Article Abstract:
A general method for quantitative comparison of biofilm structures and assessment of experimental reproducibility between independent biofilm experiments is presented with Pseudomonas aeruginosa study an example. The method depends on study using a novel computer program, COMSTAT. Structural organization of microbial communities is influenced by many factors which can be studied in this way.
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Article Abstract:
The promoter of the Paracoccus denitrificans nitric oxide (NO) reductase operon (norCBQDEF) has been studied using primer extension and deletion analysis. Two transcription-start sites were seen, the major one active only in cultures grown anaerobically, with nitrate, and is 45.5 bp downstream of the putative NNR-binding site, so this is a class II factor-dependent promoter. NNR is the sole regulatory protein with a significant role in regulating expression of the NO reductase. The NNR-binding site has been confirmed.
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Article Abstract:
Green fluorescent protein (GFP) fluorescence in Streptococcus gordonii was assessed under low-oxygen concentration and low-pH conditions. GFP, it was found, can be used in studies of oxygen-tolerant anaerobic bacteria. Densely packed, flow cell-grown biofilms of S. gordonii do not develop oxygen gradients that inhibit GFP fluorescence development. The transient nature of GFP fluorescence in acid-producing bacteria can often be overcome in flowcells.
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