Application of sequence-specific labeled 16S rRNA gene oligonucleotide probes for genetic profiling of cyanobacterial abundance and diversity by array hybridization

Article Abstract:

Diversity and distribution of cyanobacteria in planktonic communities of lakes are analyzed by Oligonucleotide probes of 16S rRNA gene of the cyanobacteria. Distribution profiles from genetic analysis correlated with those of hydrobiological and hydrochemical analysis qualitatively but relatively less at quantitative levels.

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Strain characterization and classification of oxyphotobacteria in clone cultures on the basis of 16S rRNA sequences from the variable regions V6, V7 and V8

Article Abstract:

The strain characterization and classification of oxyphotobacteria in clone cultures on the basis of 16S rRNA sequences from the variable regions V6, V7 and V8 were examined to develop a DNA-based method for characterization, classification and identification of cyanobacterial strains. The study made use of a diagnostic system using the DNA sequence polymorphism in certain 16S and rRNA regions for individual strain characterization and identification. Results show that the combination of magnetic solid-phase DNA isolation and PCR amplifications are accurate methods of classification.

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Quantification of toxic cyanobacteria in water by use of competitive PCR followed by sequence-specific labeling of oligonucleotide probes

Article Abstract:

Some of the most toxic water blooms are those formed by cyanobacteria. Therefore, its detection is important for environmental safety. In response, a nucleic-acid-based assay was developed to quantify toxic cyanobacteria in water. This assay combines sample preparation with a method of chromogenic detection based on coamplification of target and competitor DNA. Testing of the assay on the genus Microcystis yielded a detection limit of 100 cells/ml with more than three orders of magnitude of quantitative range. Its suitability for automation is an added benefit aside from sensitivity.

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