Lytic and lysogenic infection of diverse Escherichia coli and Shigella strains with a verocytotoxigenic bacteriophage

Article Abstract:

Research has been conducted on the verocytotoxigenic bacteriophage isolated from the enterohemorragic Escherichia coli strain. The use of this bacteriophage in infecting the Enterobacteriaceae strains is discussed.

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Multilocus characterization scheme for Shiga toxin-encoding bacteriophages

Article Abstract:

The PCR-based multilocus typing scheme is used for characterizing the inducible phase preparations from a variety of farm environment-derived Shiga toxin-producing Escherichia coli (STEC) lysogens. Purified Stx phages is profiled by using this scheme and the lambdoid phage-borne genes in induced STEC preparations can be identified as well as those residing in the noninducible prophage complement.

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Application of molecular biological techniques to a seasonal study of ammonia oxidation in a eutrophic freshwater lake

Article Abstract:

The application of 16S rDNA primers and probes to environmental samples and enrichment cultures at different ammonium concentrations was used in a seasonal study in which the occurrence of nitrosomonads and nitrosospiras was monitored in a eutrophic freshwater lake. Data revealed larger numbers of ammonia oxidizers and higher nitrification potentials in littoral than in profundal sediment sites. The susceptibility of littoral sediment to variation was illustrated by the fluctuation in ammonia oxidizer most-probable-numbers.

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