Application of bacteriophages to control intestinal Escherichia coli O157:H7 levels in ruminants

Article Abstract:

The characterization of O157-specific lytic bacteriophage KH1 and phage SH1 were tested, alone or in combination, for reducing intestinal Escherichia coli O157:H7 in animals. This phage therapy reduced the average number of Escherichia coli O157:H7 CFU among phage-treated steers compound to control steers, but did not eliminate bacteria from the majority of steers.

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Escherichia coli O157:H7 in environments of culture-positive cattle

Article Abstract:

The distribution and survival of Escherichia coli O157:H7 in the immediate environments of individually housed, experimentally inoculated cattle by systematically culturing feed, bedding, water, haircoat, and feed bunk walls for E. coli O157:H7 for 3 months is investigated. Survival was longest in feed at room temperature, but bacterial counts decreased over time.

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Rectal administration of Escherichia coli O157:H7: novel model for colonization of ruminants

Article Abstract:

A novel rectal swab administration of Escherichia coli O157:H7 was developed to establish experimental colonization of ruminants with this bacterium. The rectal swab administration of E. coli O157:H7 resulted in a consistent colonization of cattle and was superior to the traditional oral dosing and to the Trojan calf methods of creating experimental infections.

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