Antibacterial action of dipeptides containing an inhibitor of glucosamine-6-phosphate isomerase

Article Abstract:

The N3-(4-methoxyfumaroyl)-L-2,3-diaminopropanoic acid (FMDP) conjugated to an amino acid forms a dipeptide with significant antimicrobial properties. The dipeptide is avidly taken up by bacteria and metabolized to its components. FMDP inactivates glucosamine-6-phosphate isomerase, an enzyme necessary for the biosynthesis of a component of bacterial peptidoglycan. Inhibition of this enzyme results in bacteriolysis. FMDP is effective against both bacteria and fungi.

author: Milewski, Slawomir, Borowski, Edward, Chmara, Henryk, Andruszkiewics, Ryszard, Mignini, Fiorenzo
Enzyme inhibitors

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Efficient secretion of the model antigen M6-gp41E in Lactobacillus plantarum NCIMB 8826

Article Abstract:

Food industry research has established that the Lactobacillus strain Lb. plantarum NCIMB 8826 produces and secretes more heterologous proteins than strains Lb. paracasei LbTGS1.4, Lb. casei ATCC 393 and Lb. fermentum KLD. Lb. plantarum NCIMB 8826 was also capable of secreting a model antigen consisting of the N-terminal part of the M6 protein from bacterium Streptococcus pyogenes fused to the linear epitope ELDKWAS from human immunodeficiency virus gp41 protein.

author: Mercenier, Annick, Delcour, Jean, Hols, Pascal, Slos, Philippe, Dutot, Philippe, Reymund, Jacqueline, Chabot, Paul, Delplace, Brigitte
Food industry

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Mutations to antibiotic resistance occur during the stationary phase in Lactobacillus plantarum ATCC 8014

Article Abstract:

Lactobacillus plantarum ATCC 8014 was subjected to LCM broth, which was a nutritionally weak medium, for long periods to determine the ability of bacteria to undergo forward mutations to antibiotic resistance. Results show that mutations to antibiotic resistance in l. plantarum ATCC 8014 can take place in the absence of measurable cell division. Furthermore, results support the claim that starved bacteria in stationary phase can be genetically dynamic.

author: Collins, M.A., Thompson, J.K., McConville, K.J., McReynolds, Christine, Moorhouse, S.D.
Mutagenesis

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subjects list: Research, Antibiotics, Lactobacillus plantarum
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