Analysis of the enterohemorrhagic Escherichia coli O157 DNA region containing lambdoid phage gene p and Shiga-like toxin structural genes

Article Abstract:

A study was conducted to identify and sequence the p gene of the Shiga-like toxin(SLT)-II-converting the enterohemorrhagic Escherichia coli O157 phage 933W. The P gene sequences were used to find out the number of p genes in O157 and to study their linkage with distinct SLT genes and their restriction fragment length polymorphism. A polymerase chain reaction producing fragments hybridizing with both p and slt-I or slt-II sequences was developed.

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Presence and characterization of a mosaic genomic island which distinguishes sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- from E. coli O157:H7

Article Abstract:

A mosaic genomic island comprising Shigella resistance locus (SRL) sequences flanked by segments of Escherichia coli O157:H7 strain EDL933 O islands were identified in sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H- strain 493/89. It was found that PCR targeting the SRL- related sequence is a useful tool to distinguish SF EHEC O157:H- from EHEC 0157:H7.

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Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR

Article Abstract:

One-step multiplex PCR (MPCR) is developed and evaluated for the differential identification of intestinal pathotypes of Escherichia coli. This shows that index virulence factors are not restricted to one pathogroup but instead can also occur in other pathotypes.

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