Article Abstract:
Prolonged starvation of carbon, nitrogen, phosphorous or multiple-nutrient allows for maintenance of culturability of Vibrio vulnificus incubated at low temperature through starvation-induced protein synthesis. Carbon starvation determines the response to multiple starvation. However, nitrogen starvation results in the decline of culturability. Evaluation of culturable counts shows similar survival patterns for V. vulnificus starved for carbon, nitrogen, or phosphorous, or for all 3 nutrients simultaneously, at 24 degree celsius.
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Article Abstract:
An in situ analysis of nucleic acids in cold-induced nonculturable Vibrio vulnificus was investigated by the use of a method for assessing viability and determination of the presence of nucleic acids. Results suggest the existence of two phases of the viable but nonculturable state occurring after a loss of culturability and as a result of the gradual degradation of nucleic acids. Furthermore, viable but nonculturable cells of V. vulnificus have significant amounts of 4,6-diamidino-2-phenylindole-stainable material.
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Article Abstract:
The persistence and resuscitation potential of viable but nonculturable (VBNC) cells of the human-pathogenic bacterium Vibrio vulnificus were studied in detail by various quantitative methods. Findings revealed that VBNC cells of V. vulnificus exhibit enhanced stability after extended cold incubation, and that this resistance is similar to that of cells starved at growth temperature. VBNC cells undergo changes at low temperature which enable them to exist for extended periods.
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