Analysis of randomly amplified polymorphic DNA (RAPD) for identifying genetic markers associated with canine hip dysplasia

Article Abstract:

A study was conducted to analyze randomly amplified polymorphic DNA in locating markers that cosegregate with increases in joint laxity in domestic dogs. The Boykin spaniel was utilized as a model to study canine hip dysplasia. Results indicated that canine hip dysplasia occurs in all dog breeds and supports differences in prevalence from 3% to 70% among breeds. Findings also showed that the founder effect is a critical factor influencing abnormally high incidences of different genetic diseases.

author: Wang, X., Lepine, A.J., Miller, A.B., Scott, J.D., Murphy, K.E.
Genetic markers, Genetic disorders, Domestic animals

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Random amplified polymorphic DNA (RAPD) sequences as markers for canine genetic studies

Article Abstract:

Research was conducted to analyze the use of random amplified polymorphic DNA (RAPD) assay to genetic studies of dogs and examine the amplification of RAPD sequences with primer pairs. Polymerase chain reactions were carried out using the GeneAmp PCR System 9600. Results indicated that a novel polymorphism in the restriction digest products. Findings also suggested that RAPD can be effectively utilized as molecular markers in genetic studies of diseases in dogs.

author: Lust, G., Olivier, M., Meehl, M.A.
DNA, Chromosome mapping, Genetic polymorphisms

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An outcrossed canine pedigree for linkage analysis of hip dysplasia

Article Abstract:

A study was conducted to analyze an outcrossed canine pedigree for linkage analysis of canine hip dysplasia (CHD), a polygenic disease. Greyhounds and dysplastic Labrador retrievers were bred o determine the informative pedigree. Feeding schedules were designed to realize maximum growth rate for maximum expression of CHD. Results indicated that hip phenotype can be determined by hip joint laxity and chondro-osseous conformation.

author: Lust, G., Grohn, Y.T., Burton-Wurster, N., Acland, G.M., Olivier, M., Todhunter, T.J., Williams, A.j., Vernier-Singer, M., Farese, J.P., Gilbert, R.O., Dykes, N.L.
Dog diseases

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subjects list: Research, Genetic aspects, Dogs, Dysplasia, Hip, Diseases
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