Analysis of promoter binding by the E2F and pRB families in vivo: distinct E2F proteins mediate activation and repression

Article Abstract:

Analysis of promoter binding by the pRB and E2F and families in vivo indicates that distinct E2F proteins mediate repression and activation. A cross-linking approach in synchronized, living cells has been used to identify physiologically important E2F-responsive promoters and study their occupancy and histone acetylation state. The pattern of E2F and pRB-related polypeptides recruited to the promoters changes in a very dynamic way as the cells go from quiescence into G1 and S phase.

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Active repression and E2F inhibition by pRB are biochemically distinguishable

Article Abstract:

The retinoblastoma tumor suppressor protein can repress and inactivate independently the transcription factor E2F via targeting specific sets of transcription factors. Data indicate that the repression activity is associated with chromatin, distinguishing repression from the inactivation process.

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Culprits in the degradation of cyclin E apprehended

Article Abstract:

The degradation of cyclin E, the Cullin genes and the Cullin/CDC53 family of proteins, two members in particular, Cul3 and Cul1, are discussed in this overview article. Data show that likely multiple pathways contribute to cyclin E turnover, at least in certain types of cells. The Cul1 pathway may be more general. Unsolved questions are discussed. Analysis of cell cycle regulators will help in finding important ubiquitination pathways.

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