Analysis of a Thermotoga maritima DNA fragment encoding two similar thermostable cellulases, CelA and CelB, and characterization of the recombinant enzymes

Article Abstract:

Recombinant Escherichia coli clones exhibiting thermostable beta-glucanase activity were isolated from two different gene libraries of the hyperthermophilic bacterium Thermotoga maritima MSB8 (DSM 3109), and the nucleotide sequence of a 1,4,-beta-glucanase gene called celA was determined. CelA had a molecular mass of 29.732 kDa and was most active against soluble substrates. CelA also exhibited astonishing thermostability, which was heightened by the presence of high concentrations of salt.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Identification of genes in Rhizobium leguminosarum bv. trifolii whose products are homologues to a family of ATP-binding proteins

Article Abstract:

Nucleotide sequences of prsD, prsE (protein secretion) and orf3 from Rhizobium leguminosarum bv. trifolii TA1 were presented. The three genes were identified adjacent to the exo 133 mutation in a cosmid carrying the genomic DNA of the Rhizobium. PrsD and prsE were found homologous to the ATP-binding proteins, while orf3, which has a typical terminal sequence, was not homologous to these proteins.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

A new nos gene downstream from nosDFY is essential for dissimilatory reduction of nitrous oxide by Rhizobium (Sinorhizobium) meliloti

Article Abstract:

Research has established that the nitrogen-fixing bacteria Rhizobium (Sinorhizobium) meliloti contains seven genes in a nosRZDFY gene cluster. The cluster occurs on a 10.1 kb piece of EcoRI megaplasmid in a similar proximity to the fixGHIS genes. It takes up around 9 kb and consists of four complementation gene groups. The Hindlll-EcoRl downstream fragment is vital for nitrous oxide reduction.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: