Anaerobic transformation of furfural by Methanococcus deltae deltaLH

Article Abstract:

The Methanococcus deltae deltaLH strain synthesized furfuryl alcohol from furfural under anaerobic conditions. Incubation of Methanococcus deltae deltaLH cultures with furfural did not inhibit microbial growth. High-pressure liquid chromatography of furfural-enriched cultures also indicated the presence of furfuryl alcohol which was characterized as the final product from the anaerobic transformation of furfural. Furthermore, the reduction of furfural was mediated by aldehydes which acted as electron acceptors.

author: Boopathy, Ramaraj, Belay, Negash, Voskuilen, Gijs
Biodegradation, Methanobacteriaceae, Methanogens, Furans

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Production and purification of Remazol brilliant blue R decolorizing peroxidase from the culture filtrate of Pleurotus ostreatus

Article Abstract:

The mechanisms that mediate the H2O2-dependent Remazol brilliant blue R (RBBR) decolorizing enzymatic activity in Pleurotus ostreatus were analyzed to determine the enzyme activity involved in RBBR decolorization. The RBBR decolorizing peroxidase from Pleurotus ostreatus culture filtrates was characterized as a monomer with a native molecular mass of 73 kDa. The enzyme was also composed of 38% carbohydrates. Furthermore, RBBR peroxidase enzymatic activity had an optimal hydrogen-ion concentration or pH of 3.5.

author: Kim, Chang-Jin, Shin, Kwang-Soo, Oh, Ill-Kyoon
Fungi, Phytopathogenic, Phytopathogenic fungi, Microbial enzymes, Peroxidase

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Stereospecific biohydroxylations of protected carboxylic acids with Cunninghamella blakesleeana

Article Abstract:

Cunninghamella blakesleeana hydroxylates cycloalkylcarboxylic acids that have been protected against microbial transformation by conversion into 2-cycloalkyl-1,3-benzoxazoles. Preferential hydroxylation occurs for the carbon atoms trans to the benzoxazole group. The amounts of substrate hydrolyzed and product formed decrease with an increase in the ring size of the alkyl group. The hydroxylation of the 2-cycloalkyl-1,3-benzoxazoles produces low yield of alcohols and ketones.

author: Kreiner, M., Braunegg, G., De Raadt, A., Griengl, H., Kopper, I., Petsch, M., Plachota, P., Schoo, N., Weber, H., Zeiser, A.
Cyclic compounds, Carboxylic acids, Hydroxylation

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subjects list: Research, Microbiological synthesis, Analysis
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