An orthodenticle-related protein from Strongylocentrotus purpuratus

Article Abstract:

An orthodenticle-related protein-encoding cDNA clone SpOtx from the sea urchin Strongylocentrotus purpuratus exhibits an orthodenticle homeobox but differs from its mouse and fly equivalents in sequence. Band-shift analysis reveals that SpOtx, with a binding specificity for the TAATCC/T sequence of DNA, binds to the Spec2a gene that contains multiple sites with this motif. The dividing embryo exhibits SpOtx transcripts initially in all cells, which is limited to oral endoderm and ectoderm cells in the later stages.

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Characterization of the SpHE promoter that is spatially regulated along the animal-vegetal axis of the sea urchin embryo

Article Abstract:

A Strongylocentrotus purpuratus hatching enzyme cis regulatory region, characterized by a proximal promoter/enhancer, is found to have the ability to regulate low levels of transcriptional activity in nonvegetal cells of early sea urchin blastulae. These cis elements are Otx (an orthodenticle homologue), CCAAT, ets-related and three unidentified motifs. The subsets of these elements associate in different patterns to obtain levels of transcriptions that are compared to the unaltered regulatory region.

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A database of mRNA expression patterns for the sea urchin embryo

Article Abstract:

A study examines the genome sequencing of sea urchins by using microarray analysis.

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