Article Abstract:
An isoleucyl-tRNA synthetase gene (ileS) obtained from a Campylobacter jejuni is 2751 bp in size and similar to that obtained from E. coli. The gene is present in the 1360-1812 kb part of the genome. Isoleucyl-tRNA synthetase is an enzyme encoded by ileS gene, containing 917 amino acids with a molecular mass of 105399 Da. A comparison of the ileS gene product with that of Archea and Eukarya bacteria shows that many sequences have been conserved in these enzymes. Details of the study conducted on the gene are given.
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Article Abstract:
A lipopolysaccharide (LPS) gene cluster located on a 16 kb chromosomal DNA fragment of Campylobacter jejuni 81116 was cloned to study its involvement in LPS synthesis. Results showed that the 16kb region consists of 11 genes where the deduced proteins exhibit homology to proteins involved in LPS synthesis in other bacteria. It was also observed that the Wla genecluster contains three genes involved in the biosynthesis of sugars.
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Article Abstract:
A study was conducted to analyze the cloning, sequencing and molecular features of the Campylobacter jejuni groESL bicistronic operon. The TRIzol reagent was utilized to isolate total RNA from C. jejuni cells. Experimental results indicated that the operon was characterized by encoding proteins supporting molecular masses. Findings also showed that the operon was transcribed as a bicistronic mRNA.
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