An auxiliary mode of apoptotic DNA fragmentation provided by phagocytes

Article Abstract:

Phagocytes provide an auxiliary mode of apoptotic DNA fragmentation. Thymocytes prepared from transgenic mice that express ubiquitously a caspase-resistant form of the caspase-activated DNase (CAD) inhibitor (ICAD) have been found resistant to DNA fragmentation induced by various stimuli. It has been concluded that the DNA fragmentation that happens in apoptosis can come from CAD activity, autonomously of the cell. It could be blamed on a lysosomal acid DNase, probably DNase II, following engulfing of the apoptotic cells. CAD can bring on DNA fragmentation in apoptotic cells.

author: McIlroy, Dorian, Tanaka, Masato, Sakahira, Hideki, Fukuyama, Hidehiro, Suzuki, Misao, Yamamura, Ken-ichi, Ohsawa, Yoshiyuki, Uchiyama, Yasuo, Nagata, Shigekazu
Japan, DNA, Cell death, Phagocytes, Dornase alfa

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Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis

Article Abstract:

Bub3 gene disruption in mice has been studied. The study shows that there is an essential mitotic spindle checkpoint function in early embryogenesis. Bub3-null embryos seem to be normal to day 3.5 postcoitus. In days 4.5-6.4 they accumulate mitotic errors. Null embryos treated with a spindle depolymerizing agent do not arrest in metaphase. Mitotic disorganization is seen.

author: Kalitsis, Paul, Earle, Elizabeth, Fowler, Kerry J., Choo, K.H. Andy
Australia, Statistical Data Included, Mice, Mice (Rodents), Chromatin, Embryology, Experimental, Embryological research, Mitosis, Spindle (Cell division), Spindle (Cytoplasm)

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A murine tumor progression model for pancreatic cancer recapitulating the genetic alterations of the human disease

Article Abstract:

Research shows tumor progression leads to ductal pancreatic cancer in mice that overexpress transforming growth factor alpha (TGF-alpha). Data reveal that TGF-alpha transgenic mice rapidly develop tumors when crossbred with the tumor suppressor loci p53 suggesting involvement of other loci in the pancreatic tumor formation.

author: Wagner, Martin, Greten, Florian R., Weber, Christoph K., Koschnick, Stefan, Mattfeldt, Torsten, Deppert, Wolfgang, Kern, Horst, Adler, Guido, Schmid, Roland M.
Germany, Growth, Pancreatic cancer, Transforming growth factors, Tumors

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subjects list: Research, Usage, Physiological aspects, Genetic aspects, Cytogenetics, Genetically modified mice
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