Amplified expression of fructose 1,6-biophophatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources

Article Abstract:

The deregulated expression of fructose 1,6-biphosphatase (FBPase) in Corynebacterium glutamicum and its impact on growth and lysine production on different carbon sources are described. The overexpression of FBPase might be useful for starch- and molasses-based industrial lysine production with Corynebacterium glutamicum and the redirection of flux toward the pentose phosphate pathway (PPP) can be used in the production of other NADPH-demanding compounds as well as for products directly stemming from the PPP.

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Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose

Article Abstract:

Results reveal that optimum levels of lysine production by Corynebacterium glutamicumon fructose involves flux modification through the phosphotransferase system for fructose uptake and amplification of fructose 1,6-bisphosphatase to step-up through the pentose phosphate pathway among others. Metabolic flux analysis indicates that differences in intracellular flux depends on the carbon source of the sugars.

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Metabolic fluxes in corynebacterium glutamicum during lysine production with sucrose as carbon source

Article Abstract:

Metabolic fluxes in the central metabolism are determined for lysine producing Corynebacterium glutamicum ATCC 21526 with sucrose as a carbon source. The high TCA cycle flux and the significant secretion of dihydroxyacetone and glycerol display interesting targets to be approached by genetic engineers for optimization of the strain examined.

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