Article Abstract:
Mutants of Sulfolobus acidocaldarius that cannot tolerate high temperatures can be used to study chromosome replication, nucleoid organization, nucleoid partition and cell division. These processes are normally tightly coupled but can be uncoupled in this mutant.
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Article Abstract:
Photoreactivation was observed in unicellular organisms which included eukaryotes, bacteria and archaea. The Sulfolobus specie was used since it grows heterotrophically on different simple media, forms colonies from single cells and can produce a number of useful mutant strains. Results on the archaeon specie showed that visible light efficiently reverses the lethal effect of prior UV radiation. Photoreactivation on S. acidocaldarius was the first DNA repair process measured in archaeon, which grows optimally near 80 degrees celsius.
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Article Abstract:
A study was conducted to analyze chromosomal marker exchange in the thermophilic archaeon Sulfolobus acidocaldarius. Cell density effects were determined by varying the cell numbers on an area of 10 cm2 and the surface area where the parental strains were mated. Mixed suspension of cells were incubated to realize the effects of cell density and of parental cell ratios. Results showed that the cellular features of S. acidocaldarius mating are similar with those of Sulfolobus plasmid pNOB8.
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