Article Abstract:
Research was conducted to examine the alterations in adhesion, transport, and membrane characteristics in an adhesion-deficient mutant of Burkholderia cepacia G4, a soil pseudomonad. Comparison of the mutant designated ENV435 with the wild-type strain was performed by investigating the adhesion of the organisms to silica sand and their transport through two aquifer sediments that differed in their silt, clay and sand contents. Results indicate that differences in the cell surface polymer may cause the decreased adhesion of strain ENV435.
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Article Abstract:
Effective concentrations of plasmid encoded polychlorinated biphenyl (PCB) degradative gene products could be achieved in soil by using field application vectors (FAVs), which are a combination of a selective substrate, a host and a cloning vector. FAVs express foreign genes in nonsterile, competitive environments in which the gene products provide no advantage to the host. Such gene products are exemplified by the enzymes for the cometabolism of polychlorinated biphenyls through the bypenyl degradation pathway.
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Article Abstract:
The injection of fluorescently stained bacteria into an aquifer underlying Oyster, Virginia caused a 2- to 100-fold increase in unstained cells representing the indigenous bacteria. The detachment was not caused by increased shear forces or growth, since it occurred almost immediately after the injection.
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