Agrobacterium tumefaciens-mediated transformation of Aspergillus fumigatus: An efficient tool for insertional mutagenesis and targeted gene disruption

Article Abstract:

Transformation of Aspergillus fumigatus as a tool for insertional mutagenesis as well as for gene disruption is reported. The results suggested that Agrobacterium tumefaciens-mediate transformation (ATMT) is an efficient tool for transformation, random insertional mutagenesis, and gene disruption in Aspergillus fumigatus.

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The Ti plasmid of Agrobacterium tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-acyl homoserine lactonase activity

Article Abstract:

Research has been conducted on Agrobacterium tumefaciens C58 genome containing N-acyl homoserine lacton hydrolases. Results demonstrate that after being expressed in Escherichia coli two N-acyl homoserine lactone hydrolases confer the ability to degrade N-acyl homoserine lactone hydrolases on the host.

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Greater diversity of Shiga toxin-encoding bacteriophage insertion sites among Escherichia coli O157:H7 isolates from cattle than in those from humans

Article Abstract:

A study was conducted to characterize the bacteriophage insertion site genotype of Escherichia coli O157:H7 isolates from cattle and from humans and found increased genotype diversity among bovine isolates and differential representation of genotypes in these host species.

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