Aeromonas hydrophila isolated from food and drinking water: hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (HT-29)

Article Abstract:

Aeromonas hydrophila-mediated hemolysis takes place more in water isolates than in food isolates, whereas hemagglutination is more frequent in food isolates than in water isolates. Cytotoxicity of the bacterium is equally prominent in food and water isolates. These results are based on a study for the pathogenicity of Aeromonas hydrophila in the erythrocytes of human intestinal cell line (HT-29) and six other cells. Findings suggest that use of the HT-29 cells is effective for studying the enteropathogenicity of the bacterium for humans.

author: Handfield, Martin, Simard, Peirre, Couillard, Michel, Letarte, Robert
Research, Analysis, Physiological aspects, Intestines, Hemolysis and hemolysins, Hemolysis, Blood, Blood typing

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Highly sensitive protein translation assay for trichothecene toxicity in airborne particulates: comparison with cytotoxicity assays

Article Abstract:

Trichotecene and air particulate samples from fungus-contaminated houses are used in comparing the performance of a new nonradioactive assay based on translation of firefly luciferase in a rabbit reticulocyte system with that of the MTT assay. The activity of the translated luciferase is measured directly through a luminometer. Results reveal that the new assay enjoys a 400-fold increase in sensitivity of trichotecene detection against the MTT assay. The former also has a higher specificity for trichotecenes than the latter.

author: Dearborn, Dorr G., Yike, Iwona, Allan, Terry, Sorenson, William G.
Evaluation, Equipment and supplies, Measurement, Environmental monitoring, Microbiological assay, Trichothecenes

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Identification of motile Aeromonas strains with the MicroScan WalkAway system in conjunction with the Combo Negative type 1S panels

Article Abstract:

The MicroScan WalkAway system in conjunction with the Combo Negative type 1S panels identified 80% of 85 strains of Aeromonas from eight different species. This system is faster and less labor-intensive than conventional biochemical methods.

author: Vivas, J., Saa, A.I., Tinajas, A., Barbeyto, L., Rodriguez, L.A.
Identification and classification

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subjects list: Bacteria, Pathogenic, Pathogenic bacteria
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