Adaptation of psychrophilic and psychrotrophic sulfate-reducing bacteria to permanently cold marine environments

Article Abstract:

Bacteria adapt to low temperature conditions on long-term incubations. A study of the ability of bacteria to reduce sulfate in two marine sediments in Denmark and Antarctica indicates that the psychrotrophic bacteria grow optimally between 18 and 19 degrees celsius during short-term incubations. Incubations for a week reduce the optimum temperature to 12.5 degrees celsius. The psychrotrophic strains of bacteria reduce sulfate optimally at 28 degrees celsius in short-term incubations. The psychrophilic strains have an optimum growth under a temperature between 30 to 35 degrees celsius.

author: Jorgensen, Bo Barker, Isaksen, Mai Faurschou
Observations, Cold adaptation

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Marine Vibrio species produce the volatile organic compound acetone

Article Abstract:

Examination of different aerobic, heterotrophic marine bacteria for production of volatile organic compounds enables the identification of a group of isolates, which produce significant amounts of acetone. Nonclinical Vibrio species are the major producers of acetone and chromatography confirms the production of acetone. Addition of L-leucine increases the production of acetone, suggesting that formation of acetone involves the degradation of leucine. Production of acetone is maximum during the stationary phase of growth.

author: Fall, Ray, Nemecek-Marshall, Michele, Wojciechowski, Cheryl, Kuzma, Jennifer, Silver, Gary M.
Microbiological synthesis

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Characterization of a Pseudomonas putida allylic alcohol dehydrogenase induced by growth on 2-methyl-3-buten-2-ol

Article Abstract:

The soil bacterium Pseudomonas putida was isolated to develop an enzymatic assay for the alcohol 2-methyl-3-buten-2-ol (232-MB) which is produced and emitted by certain pines. P. putida uses 232-MB as a sole carbon source. Analysis of components of strain MB-1 suggests that 232-MB is metabolized by isomerization to 321-MB followed by oxidation. The N-terminal sequence of the 321-MB dehydrogenase contained a region of 64% identity with the TOL plasmid-encoded benzyl alcohol dehydrogenase of P. putida.

author: Fall, Ray, Nemecek-Marshall, Michele, Malone, Vincent F., Chastain, Amy J., Ohlsson, John T., Poneleit, Loelle S.
Soil microbiology, Pseudomonas putida, Alcohol dehydrogenase, Alcohol dehydrogenases, Microbiological assay

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subjects list: Research, Marine bacteria
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