Article Abstract:
Three Pseudomonas stutzeri strains were examined to determine the arrangement of the genes involved in o-xylene, m-xylene and p-xylene catabolism. The strains are the wild-type strain OX1, the mutant M1 and the revertant R1. Results revealed the presence of a 3-kb insertion sequence (IS) called ISPs11, which inactivates the m-xylene and p-xylene catabolic pathway in P stutzeri OX1 and the o-xylene catabolic genes in Pstutzeri M1. There was no IS detected in the corresponding catabolic regions of the P stutzeri R1 genome, but ISPs1 was detected in several copies in the genomes of the three strains.
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Article Abstract:
The nucleotide sequence of the gene cluster coding for the toluene/o-xylene monooxygenase cloned from Pseudomonas stutzeri OX1 was determined and characterized. Results revealed the presence of six open reading frames homologous to other genes clustered in operons coding for multicomponent monooxygenases found in benzene- and toluene-degradative pathways cloned from Pseudomonas strains. Similarities were found with multicomponent monooxygenase systems for phenol, methane, alkene and dimethyl sulfide cloned from various bacterial strains.
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Article Abstract:
Mutant M1 has been isolated from Pseudomonas stutzeri strain OX1 which could thrive on m- and p-xylenes but not on o-xylene. The m- and p-xylenes were reduced through the progressive oxidation of a methyl group. A new revertant strain called R1 has been isolated from M1 that thrives on the m-, p- and o-xylenes. The o-xylene is reduced by two consecutive monooxygenations of the aromatic ring while the m- and p-xylenes are reduced by the progressive oxidation of a methyl substituent.
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