Acquisition of a deliberately introduced phenol degradation operon, pheBA, by different indigenous Pseudomonas species

Article Abstract:

A research was conducted to investigate the acquisition of a deliberately released phenol degradation open by the indigenous microflora from river water polluted by phenolic compounds. P. putida and Escherichia coli were utilized as DNA recipient strains while pseudomonads were cultured at 30 degrees C. Results indicated that the expression of phe genes depended on the genetic background of the hosts. The dilution of the leachate water with river water contributed to the high efficiency of the natural biodegradation.

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Small-scale DNA sample preparation method for field PCR detection of microbial cells and spores in soil

Article Abstract:

The construction of a small-scale, non-selective procedure for DNA sample preparation supporting rapid-in-the-field polymerase chain reaction analysis for detection of microbial spores and cells is described. Three methods capable of releasing genomic DNA from three microorganisms were chosen for cell and spore disruption techniques. The reagents and buffers used in the three methods were changed and the techniques are miniaturized to provide maximum DNA yields.

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Generation of novel bacterial regulatory proteins that detect priority pollutant phenols

Article Abstract:

Researchers have created a Pseudomonas strain that can be used as a biosensor for phenols. This is done by placing a reporter gene under the control of a promoter that is induced by phenol or any other pollutant.

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