Accumulation of trehalose by overexpression of tps1, coding for trehalose-6-phosphate synthase, causes increased resistance to multiple stresses in the fission yeast Schizosaccharomyces pombe

Article Abstract:

Modulation of the function of the tps1 gene in the fission yeast Schizosaccharomyces pombe considerably affects tolerance not only of temperature upshifts but also of other stresses. These stresses include freezing and thawing, dehydration, and growth in the presence of normally toxic concentrations of ethanol and NaCl. This was found following an evaluation of the role of trehalose in protection against stress-induced injuries by inducing overexpression, under normal physiological conditions, of tps1, which codes for trehalose-6P synthase in cells of S pombe. This approach allowed the manipulation of trehalose levels without prestressing the cells and exhibits a central role for trehalose in fission yeast stress response.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Purification and characterization of two dihydroxyacetone kinases from Schizosaccharomyces pombe IFO 0354

Article Abstract:

The characterization of two dihydroxyacetone kinases (DHAKs), DHAK I and DHAK II, purified from Schizosaccharomyces pombe IFO 0354, shows that they are immunologically different from each other. DHAK II is more important than DHAK I for the dissimilation of glycerol via dihydroxyacetone. When the enzyme activity is measured in a mixed buffer, DHAK I shows optimum activity at pH 6.0 and DHAK II at pH 7.0. The optimum temperature for the activity of DHAK I is 50 degree celsius (C)and that for the activity for DHAK II is 60 degree C.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Influence of polyethylene glycol on the size of Schizosaccharomyces pombe electropores

Article Abstract:

Exposure of Schizosacchromyces pombe cells to polyethylene glycol (PEG) results in increase cell permeability. This was based on flourescein isothiocyanate-dextran uptake and transformation studies which showed enlargement of membrane electropores upon incubation with PEG. This enlargement led to increased permeability. Exactly how this change in electrophores occur is still unknown. Heat pulse transformation studies indicate that PEG might also be involved in macromolecular cell uptake but the mechanism is likewise undetermined.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: