Accumulation of copper and other metals by copper-resistant plant-pathogenic and saprophytic pseudomonads

Article Abstract:

The copper accumulation in copper-resistant strains Pseudomonas species was determined. The bacteria were grown in MGY broth containing concentrations of metal salts, harvested by centrifugation, and freeze-dried then metal content was analyzed by atomic absorption spectrophotometry. Results showed that copper resistance in Psedumonas species is caused by copper sequestration, a mechanism which is common to Pseudomonas species found on plants to which antimicrobial copper was applied. Further tests also showed that P. syriangae strain PS61 containing cop operon accumulated more copper than PS61 without the operon.

author: Cooksey, Donald, Azad, Hamid R.
Pseudomonas syringae

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Influence of volatile fatty acids on nitrite accumulation by a Pseudomonas stutzeri strain isolated from a denitrifying fluidized bed reactor

Article Abstract:

Pseudomonas stutzeri cells accumulate nitrite when incubated with acetate or propionate, but not when incubated with butyrate or valerate. The difference in the effect of volatile fatty acids on nitrite accumulation is due to differences in the flow of electrons to cytochrome c. Nitrite accumulation in acetate-incubated cells is due to variable rates of nitrate and nitrite reduction and competition for electrons between the two reduction pathways. Butyrate decreases nitrate reduction and increases the reduction of nitrite. Nitrate suppresses nitrite reduction only in acetate-incubated cells.

author: Barak, Yoram, Tal, Yossi, Van Rijn, Jaap
Fatty acids, Nitrites, Bioaccumulation, Acetates

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Contrasting effects of a nonionic surfactant on the biotransformation of polycyclic aromatic hydrocarbons to cis-dihydrodiols by soil bacteria

Article Abstract:

The biotransformation of naphthalene and phenanthrene by the diogenase-expressing bacteria Pseudomonas sp. strain 9816/11 and Sphingomonas yanoikuyae B8/36 under limited substrate oxidation has been studied. The strains are mutants that can accumulate cis-dihydrodiol metabolites under the experimental conditions employed. The roles of the nonpolar solvent 2,2,4,4,6,8,8-heptamethylnonane and the nonionic surfactant Triton X-100 on the rate of accumulation of the metabolites have also been investigated.

author: Larkin, Michael J., Allen, Christopher C.R., Boyd, Derek R., Hempenstall, Francis, Sharma, Narain D.
Environmental aspects, Soil microbiology, Polycyclic aromatic hydrocarbons, Surface active agents

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subjects list: Physiological aspects, Pseudomonas, Research
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