Article Abstract:
A survey of environmental E. coli isolates from recreational waters was carried out for the first time using a DNA microarray capable of detecting all currently described virulence genes and commonly found antimicrobial resistance genes. A high proportion (29%) of 308 isolates from a beach site in the Great Lakes carried a pathotype set of virulence-related genes and 14% carried antimicrobial resistance genes, findings consistent with a potential risk for public health.
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Article Abstract:
The detection efficacy of an oligonucleotide-based microarray designed with probes specific for the universal targets of 16S rRNA and cpn60 genes in addition to the Enterobacteriaceae-specific wecE genes of several pathogens usually encountered in wastewater is assessed. The results show the feasibility of using DNA microarrays in the detection of waterborne pathogens within mixed populations but also raise the problem of PCR bias in such experiments.
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Article Abstract:
An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance genes is designed and evaluated using DNA from known reference strains. This microarray is a powerful diagnostic tool for monitoring emerging E. coli pathotypes and antimicrobial resistance, as well as for environmental, epidemiological and phylogenetic studies including the evaluation of genome plasticity.
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