Low-temperature lipase from psychrotrophic Pseudomonas sp. strain KB700A

Article Abstract:

Research has been conducted on the psychrotrophic bacteria Pseudomonas sp. strain KB700A which produces a lipase. The isolation of the psychrotrophic strain KB700A from a subterranean environment has been carried out, and the results indicate that the lipase gene from this strain has been identified and that its gene product has been characterized.

author: Imanaka, Tadayuki, Rashid, Naeem, Shimada, Yuji, Ezaki, Satoshi, Atomi, Haruyuki
Genetic aspects, Pseudomonas, Lipase, Microbiological research, Low temperatures, Psychrotrophic organisms, Psychrotrophic microorganisms

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Engineering of a type III rubisco from a hyperthermophilic archaeon in order to enhance catalytic performance in mesophilic host cells

Article Abstract:

The different ways for improving the function of type III ribulose 1,5-bisphosphate carboxylase/oxygenase (Rbctk) at close temperature is analyzed. Results conclude that Loop 6 plays a crucial role in the turnover and specificity of type I and type II rubiscos.

author: Imanaka, Tadayuki, Atomi, Haruyuki, Yoshida, Shosuke
Science & research, Nucleic acids, Properties, Western immunoblotting, Western blot, Nucleic acid isolation, Rubisco, Hyperthermophiles

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A unique chitinase with dual active sites and triple substrate binding sites from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1

Article Abstract:

A unique chitinase has been found to come from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 and its unique gene has been cloned and sequnced. The chitinase has dual active sites and triple substrate binding sites.

author: Takagi, Masahiro, Imanaka, Tadayuki, Tanaka, Takeshi, Fujiwara, Shinsuke, Shingo, Nishikori, Jukui, Toshiaki
Enzymes, Bacteria, Thermophilic, Chitin, Cytochemistry, Binding sites (Biochemistry), Active sites (Biochemistry), Thermophiles

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subjects list: Statistical Data Included, Analysis, Japan, Physiological aspects, Research
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