A simple and reliable method for hybridization of homothallic wine strains of Saccharomyces cerevisiae

Article Abstract:

A study was conducted to characterize a technique for homothallic wine yeast spore hybridization that supports hybrid selection and identification. The method accommodates a killer phenotype common in wine yeasts. The killer phenotype of all colonies isolated from each cross were analyzed for their ability to generate K+ CYH hybrids. Results indicated that the hybridization technique can be utilized to support genetic studies of natural wild yeast populations without crossing laboratory domesticated strains.

author: Ramirez, Manuel, Perez, Francisco, Regodon, Jose A.
Yeast, Saccharomyces, Yeast (Food product), Hybridization

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Correlation of functional instability and community dynamics in denitrifying dispersed-growth reactors

Article Abstract:

A study was conducted to examine the community dynamics of two dispersed-growth denitrifying reactors during period of functional stability and instability. The results reveal a clear relationship between community dynamics and functional instability and the importance of diversity among nitrate-reducing populations within a denitrifying community.

author: Gentile, M.E., Jessup, C.M., Nyman, J.L., Criddle, C.S.
Denitrification

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Genetic instability of heterozygous, hybrid, natural wine yeasts

Article Abstract:

The genetic instability in natural wine yeasts and hybrids synthesized in the laboratory due to loss-of-heterozygosity (LOH) at the cyh2 locus were characterized and quantified. The LOH phenomenon is economically important if it causes sudden phenotype changes in industrial and pathogenic yeasts.

author: Ramirez, Manuel, Vinagre, Antonia, Ambrona, Jesus, Molina, Felipe, Maqueda, Matilde, Rebollo, Jose E.
Analysis, Wine microbiology, Heterozygosis, Heterozygote

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subjects list: Research, Biological diversity, Biodiversity
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