Article Abstract:
A genetic map of Cochliobolus heterostrophus, the southern corn leaf blight pathogen, was developed using restriction fragment length polymorphism and pulsed field gel electrophoresis analyses. The results showed that the total map length of C. heterostrophus is about 1,501 cM, with the current map covering 63% of the total length. Sixteen chromosomes were detected in one strain, while others typically contained 15 chromosomes. The sixteenth chromosome appears to be a B chromosome, without any essential function. The present map will be useful in facilitating the study of fungal pathogenesis in plants.
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Article Abstract:
Several Arabidopsis thaliana were used in developing a method for genome-wide high-resolution genetic linkage mapping. The results showed that progenies of homozygous parents contained more genetic information on recombination than their parents or recombinant inbred populations. It was concluded that the method, which uses progenies instead homozygous parents, is more suited in genome-wide genetic linkage mapping.
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Article Abstract:
Multiple markers associated with the centromeres are investigated by a maximum likelihood method. This method also enables the determination of recombination frequencies between centromeres, markers and marker loci. The method is coupled with oocyte and polar body typing methods for effective gene-centromere mapping. The new technique also yields female linkage maps.
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