A real-time PCR assay for the detection of Campylobacter jejuni in foods after enrichment culture

Article Abstract:

Research describes the development of a real-time polymerase chain reaction assay based on 5' nuclease system to detect specific C sequence in Campylobacter jejuni. The assay shows linearity of quantification of food contamination over six orders of magnitude, detecting up to 12 genome equivalents. The new assay takes 3 h and applicable to a diverse range of Campylobacter species and other related and unrelated genera.

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Detection of Clostridium proteoclasticum and closely related strains in the rumen by competitive PCR

Article Abstract:

A competitive polymerase chain reaction (PCR) procedure which detects the proteolytic bacterium Clostridium proteoclasticum and these closely related Butyrivibrio fibrisolvens strains directly from the rumen was developed. The cPCR approach is found to be an easy and accurate method of bacterial quantitation. It was also found to be useful in quantifying ruminal bacterial populations in vivo.

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Detection of Salmonella enterica serovar Typhimurium by using a rapid, array-based immunosensor

Article Abstract:

Research discuses the results of a rapid, multianalyte array biosensor testing of Salmonella in food samples and the test's usage in preharvest testing of chickens for Salmonella infection. Results indicate that that the detection limit of the test is 8 X 10(sup)4 CFU/milliliter.

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