A putative cyclic peptide efflux pump encoded by the TOXA gene of the plant-pathogenic fungus Cochliobolus carbonum

Article Abstract:

The TOXA gene of the plant-pathogen fungus, Cochliobolus carbonum, encodes an HC-toxin efflux pump which plays a role in self-protection against HC-toxin. The gene is present only in the fungal isolates that make HC-toxin, and it occurs as two linked copies. TOXA occurs close to HTS1, the gene found in HC-toxin making fungal isolates, that codes for the enzyme necessary for the synthesis of HC-toxin. The amino acid sequence of TOXA gene is discussed.

author: Panaccione, Daniel G., Pitkin, John W., Walton, Jonathan D.
Fungi, Microbial toxins

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Targeted mutants of Cochliobolus carbonum lacking the two major extracellular polygalacturonases

Article Abstract:

Research was conducted to examine the targeted mutants of Cochliobolus carbonum lacking the two major extracellular polygalacturonases (exoPG). The purification of the exoPG activity of C carbonum and the cloning of its gene, PGX1, was also performed. Double pgn1/pgx1 mutant strain was developed by crossing. The continued growth of the pgn1/pgx1 mutant strain on pectin could be attributed to one or more of PG residual activities.

author: Pitkin, John W., Walton, Jonathan D., Scott-Craig, John S., Cheng, Yi-Qiang, Cervone, Felice, De Lorenzo, Giulia
Pectin, Microbial enzymes, Microbial mutation

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A putative branched-chain-amino-acid transaminase gene required for HC-toxin biosynthesis and pathogenicity in Cochliobolous carbonum

Article Abstract:

Results reveal that TOXF gene has a role in the HC-toxin production and pathogenicity of Cochliobolus carbonum. The amino acid sequence data show homology to branched-chain-amino-acid transaminases from other species.

author: Walton, Jonathan D., Cheng, Yi-Qiang, Ahn, Joong-Hoon
United States, Physiological aspects, Bacterial toxins, Branched chain amino acids

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subjects list: Research, Analysis, Amino acid sequence, Amino acid sequencing
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