Article Abstract:
The TOXA gene of the plant-pathogen fungus, Cochliobolus carbonum, encodes an HC-toxin efflux pump which plays a role in self-protection against HC-toxin. The gene is present only in the fungal isolates that make HC-toxin, and it occurs as two linked copies. TOXA occurs close to HTS1, the gene found in HC-toxin making fungal isolates, that codes for the enzyme necessary for the synthesis of HC-toxin. The amino acid sequence of TOXA gene is discussed.
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Article Abstract:
Research was conducted to examine the targeted mutants of Cochliobolus carbonum lacking the two major extracellular polygalacturonases (exoPG). The purification of the exoPG activity of C carbonum and the cloning of its gene, PGX1, was also performed. Double pgn1/pgx1 mutant strain was developed by crossing. The continued growth of the pgn1/pgx1 mutant strain on pectin could be attributed to one or more of PG residual activities.
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Article Abstract:
Results reveal that TOXF gene has a role in the HC-toxin production and pathogenicity of Cochliobolus carbonum. The amino acid sequence data show homology to branched-chain-amino-acid transaminases from other species.
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