Article Abstract:
Previous research has isolated two regions in the Methylobacterium extorquens AM1 DNA code that complement three mutants blocked in their ability to convert acetyl-CoA into glyoxylate, which is required to assimilate methanol and ethanol. Another region with the same characteristic and containing two ORFs and the 3'-end of a third one is isolated and sequenced. The first ORF, dubbed meaA, maps all mutations in the three mutants, the second encodes a polypeptide that is unlike any known protein while the partial ORF encodes a protein similar to 3-oxoacyl-[acyl-carrier-protein] reductases.
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Article Abstract:
Periplasmic transport systems are synthesized in methylotrophic bacteria in steps that involve the identification of preprotein, its transport to the periplasm and addition of the methanol oxidation system. Preproteins with alpha-helical structures are tagged by a signal peptide such as a lipoprotein, brought to the periplasm by secretory or ribonucleoprotein pathways and folded into the periplasm. The methanol oxidation system is formed from pyrroquinoline quinone and a cytochrome.
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Article Abstract:
Research indicates that enteric bacterium Klebsiella pneumoniae uses formate dehydrogenase-O in aerobic respiration and microaerobic nitrogen fixation via the terminal oxidase enzyme cydAB. Evidence suggests that formate dehydrogenase-O enzyme passes electrons to a respiratory chain. The gene sequence of cydAB is established.
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