A primer on vesicle budding

Article Abstract:

A model for transport vesicle formation is reported. This model revolves around the priming complex, which is formed by a primer protein, a small GTPase, and one or more subunits of the vesicular coat, including a protein that activates GTPase. It is theorized that vesicle formation occurs in four stages: GTPase activation and membrane binding, priming complex formation, coat oligomerization and loss of the GTPase, and vesicle budding.

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Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins

Article Abstract:

A mammalian Pins, called LGN, binds G alpha i and interacts through an N-terminal domain with the microtubule binding protein NuMA. A FRET biosensor is used in a study to demonstrate that LGN acts as a conformational switch in its closed state, the N and C termini interact, but NuMA or G alpha i can disrupt this association, allowing LGN to interact simultaneously with both proteins, resulting in their cortical localization.

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Closing the GAP between polarity and vesicle transport

Article Abstract:

Polarity proteins may be required for the establishment of tight junctions and transmembrane proteins of the tight junctions must be delivered to the plasma membrane in vesicles. Evidence is provided for a new pathway that links polarity proteins and vesicle transport to the maintenance of tight junctions, through the control of Cdc42 by Rich1, a GTPase-activating protein.

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