A gene encoding a novel multidomain beta-1,4-mannanase from caldibacillus cellulovorans and action of the recombinant enzyme on kraft pulp

Article Abstract:

Results show that the multidomain beta-1,4-mannanase gene consists of 4,567 base pair nucleotide sequence and 3 open reading frames. Data also indicate that the recombinant mannases along with beta-xylanase increase the brightness of the bleached pulp.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

A novel thermostable multidomain 1,4-beta-xylanase from 'Candibacillus cellulovorans' and effect of its xylan-binding domain on enzyme activity

Article Abstract:

Research describes cloning, sequencing, and expression of the10 beta-xylanase gene from a new thermophilic spore producing aerobic bacterium 'Candibacillus cellulovorans'. Data show that the gene encoded protein exhibits a single xylan-binding domain and two cellulose-binding domains and absence of the former affects the thermostability of the enzyme's catalytic domain.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Cloning of the xynB gene from Dictyoglomus thermophilum Rt46B.1 and action of the gene product on kraft pulp

Article Abstract:

A second xylanase gene (xynB) from Dictyoglomus thermophilum Rt46B.1 was identified using a two-step polymerase chain reaction (PCR) protocol, cloned and expressed. The two-step PCR consisted of a consensus PCR to identify any family 11 xylanase genes in the Rt46B.1 genome and a genomic walking PCR to sequence family 11 xylanases. The XynB xylanase was able to enhance the bleachability of eucalypt kraft-oxygen pulp in both elemental chlorine-free (ECF) and total chlorine-free bleaching and that of Pinus radiata kraft-oxygen pulp in ECF bleaching.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: