A novel role for Xist RNA in the formation of a repressive nuclear compartment into which genes are recruited when silenced

Article Abstract:

Examination of the three-dimensional organization of X-linked genes reveals that when transcribed, they are always located at the periphery of, or outside, the Xist RNA domain, in contact with the transcription machinery. Upon silencing, gene shifts to a more internal location, within the Xist RNA compartment devoid of transcription factors and the appearance of this compartment is not dependent on the A-repeats of the Xist transcript, which are essential for gene silencing.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Tsix-mediated epigenetic switch of a CTCF-flanked region of the Xist promoter determines the Xist transcription program

Article Abstract:

A precisely defined Xist promoter region flanked by CTCF is maintained by Tsix in a heterochromatic-like state in undifferentiated embryonic stem (ES) cells and shifts to a pseudoeuchromatic structure upon Tsix truncation is shown. It is observed that the Tsix mediated epigenetic regulation of Xist, which resulted in Xist promoter activation, and initiation of X inactivation, in differentiating ES cells.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Tsix transcription across the Xist gene alters chromatin conformation without affecting Xist transcription: Implications for X-chromosomes inactivation

Article Abstract:

A demonstration that RNA polymerase II preintiation complex recruitment and H3 Lys 4 (H3-K4) methylation at the Xist promoter form the basis of the Xist expression profiles that drives both imprinted and random X-chromosome inactivation (XCI) is presented. X-chromosome inactivation (XCI) is highly dynamic during early mouse embryogenesis and strictly depends on the Xist noncoding RNA.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: