Article Abstract:
A study was conducted to analyze the use of grass clippings discharged from golf courses as the raw material for the production of a suppressive compost to control Rhizoctonia large-patch disease in mascarene grass. Bacillus subtilis N4 was utilized as an inoculum in a process designed to control composting temperatures and inoculation timing. Results indicated that the production of high concentrations of disease-suppressive bacteria in compost correlated with the concentration of other bacteria present in the raw material.
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Article Abstract:
The identification of additional non-Acremonium sp. grass endophytes, designated p-endophytes, was reported. Both the Phialophora-like and Gliocladium-like endophytes were cosymbiotic with Acremonium endophytes in their host grasses. It was shown that similar cosymbiotic associations exist in three more grass species: Festuca arizonica, Festuca gigantea and Festuca pratensis. Serological and DNA sequence data indicate a close relationship between the two non-Acremonium endophytes and the p-endophytes.
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Article Abstract:
A PCR assay which can be used for the detection and quantification of Epichloe endophytes in tissues of the grass Bromus erectus is presented. Findings revealed that the microsatellite locus may be useful for in planta detection of a variety of related species, including agronomically important Acremonium coenophialum and Acremonium lolii. No fragments were produced from DNA isolates from uninfected plant material or from unrelated fungi isolated from B. erectus.
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