A new biocatalyst for production of optically pure aryl epoxides by styrene monooxygenase from Pseudomonas fluorescens ST

Article Abstract:

A novel biocatalyst for synthesizing optically pure aryl epoxides has been developed by cloning the styrene monooxygenase genes styA and styB from Pseudomonas fluorescens ST. Several aryl vinyl and aryl ethenyl derivatives were oxidized by recombinant Escherichia coli to their respective optically pure epoxides. The bioconversions suggest that styrene monooxygenase exhibits a broad substrate preference as well as a potential for the formation of different fine chemicals.

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Induced reporter gene activity, enhanced stress resistance, and competitive ability of a genetically modified Pseudomonas fluorescens strain released into a filed plot planted with wheat

Article Abstract:

The Pseudomonas fluorescens RIWE8 mutant was analyzed to determine the effects of lacZ genetic modification on the ecological behavior of Pseudomonas fluorescens. Pseudomonas fluorescens R2f and RIWE8 strains grown in field soil microplots indicated the presence of higher reporter gene activity in wheat rhizosphere with RIWE8 strains. However, the genetically modified strains and the parental strain exhibited minor differences in their ecological properties.

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Specific detection and real-time PCR quantification of potentially mycophagous bacteria belonging to the genus Collimonas in different soil ecosystems

Article Abstract:

The use of Collimonas-specific restriction fragment length polymorphism (RFLP) assay and real-time PCR protocol for specific detection of collimonads among culturable chitin-degrading soil bacteria and in total soil DNA respectively is discussed. PCR protocol resulted in the detection of Collimonas in 80 percent of the soil samples.

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