A natural view of microbial biodiversity within hot spring cyanobacterial mat communities

Article Abstract:

The microbial ecology occurring in the hot spring cyanobacterial mat community found in the effluent channels of the October Spring at the Yellowstone National Park was studied through the 16S rRNA-based cloning, sequencing and oligopeptide probe methods. Results were obtained through the denaturing gradient gel electrophoresis analysis. It was discovered that cyanobacterial populations adapted to narrow temperature-defined niches along the estimated 30 degrees temperature range. Light intensity was the most apparent predictor of changing environmental parameter.

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Denaturing gradient gel electrophoresis used to monitor the enrichment culture of aerobic chemoorganotrophic bacteria from a hot spring cynobacterial mat

Article Abstract:

The denaturing gradient gel electrophoresis separation of 16S rRNA gene fragment helps to study the effect of enrichment culture techniques on the composition of the aerobic chemoorganotrophic bacteria population in Octopus Spring, Yellowstone National Park. Variations in the enrichment conditions, degree of dilution of the inoculum and culture medium composition give 14 unique 16S rRNA sequences. The results reveal the diversity in bacterial populations obtained by enrichment methods in contrast to molecular retrieval and strain purification techniques.

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Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription-PCR in temperature gradient gel electrophoresis fingerprints

Article Abstract:

The major bacterial 16S rRNAs in Drentse A grassland soils in the Netherlands were amplified by reverse transcription-polymerase chain reaction (RT-PCR) with bacterium-specific primers and were differentiated by temperature gradient gel electrophoresis. The sequence-specific efficiency of amplification was measured by competitive PCR and RT-PCR, and identical input quantities of different 16S rRNAs produced identical amplicon yields.

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