A mutant of Listeria monocytogenes L028 unable to induce an acid tolerance response displays diminished virulence in a murine model

Article Abstract:

A research was conducted to study the ability of a Listeria monocytogenes L028 mutant to induce an acid tolerance response (ATR) using log-phase cultures centrifuged in TSB-YE preheated to either 42 degrees C or 37 degrees C. The acid-adapted L028 was showed to exhibit cross-protection against other stresses while the ATR1 cells which reached the spleens had a growth rate similar to that of the parent cells. Moreover, the mutant showed a capability to adapt to acid stress which is important for the pathogenesis of L. monocytogenes.

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Bile salt hydrolase activity in probiotics

Article Abstract:

The ecological significance of bile salt hydrolase (BSH) production is explored and the impact that bile hydrolysis may have on human physiology is examined. It is shown that bile salt hydrolase (BSH) enzymes might confer a selective advantage on probiotic strains in the highly competitive environment of the human intestinal tract.

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Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria

Article Abstract:

A novel vector is constructed for the constitutive luminescent tagging of gram-negative bacteria by using site-specific integration into the 16S locus of the bacterial chromosome. Many gram-negative pathogens are successfully tagged by using this vector and this system is validated during murine infections of living animals.

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