Article Abstract:
Research reveals that albicidin biosynthesis is mediated by the polyketide synthase that is linked to a non-ribosomal peptide synthetase encoded by the xabB gene in Xanthomonas albilineans. Data show that the multifunctional enzyme is the largest reported polyketide- peptide synthetase and contains twelve catalytic domains.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
Soil isolates of Alcaligenes denitrificans express the albicidin resistance gene albB that codes for a 23 kDa protein that detoxifies albicidin through reversible binding. Partial DNA sequence homology is present between regions of the 621 bp coding domain and the albicidin resistance gene albA of Kelbsiella oxytoca. The albicidin resistance AlbR proteins from the two bacteria exhibit N-terminal homology but utilize varying codons for shared amino acids. Detection analysis, albicidin binding by a synthetic oligopeptide and translational fusions indicate the N-terminal region as a functional domain.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
The albA gene from Klebsiella oxytoca encodes a protein which inactivates albicidin through heat-reversible binding. Albicidin ligand binding to a recombinant AlbA protein, purified by a glutathione S-transferase gene fusion system, is an almost instant and saturable reaction. Kinetic and stoichiometric studies of the binding reaction showed the presence of one high affinity binding site with a dissociation constant of 6.4 x 10(super -8) M. AlbA may be a useful affinity matrix for selective purification of albicidin antibiotics.
User Contributions:
Comment about this article or add new information about this topic: