A most-probable-number assay for enumeration of infectious Cryptosporidium parvum oocysts

Article Abstract:

Research was conducted to quantify infectious Cryptosporidium parvum oocysts using the most-probable-number (MPN) method of enumerating microorganisms. The approach involved creating a dilution series, incubating the samples for an adequate period, plating in replicate and scoring the presence of growth. Results provide evidence that the focus detection method-MPN approach is an excellent and reproducible assay for quantifying oocyst infectivity in vitro.

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Use of cell culture to assess Cryptosporidium parvum survival rates in natural groundwaters and surface waters

Article Abstract:

A study is conducted to determine the survival rates of Cryptosporidium parvum in surface waters and ground-waters of Florida by using cell culture. Findings reveal that temperature, surface water or groundwater type, and the interaction of these factors has statistically significant effects on the survival of Cryptosporidium parvum.

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Infectious Cryptosporidium parvum oocysts in final reclaimed effluent

Article Abstract:

The authors have investigated water samples collected from reclamation facilities for the presence of Cryptosporidium parvum. They report that the focus detection method-most-probable-number cell culture technique has revealed the presence of infectious C. parvum in the final disinfected effluent samples.

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