A modified Saccharomyces cerevisiae strain that consumes L-arabinose and produces ethanol

Article Abstract:

Research describes metabolic engineering of a Saccharomyces cerevisiae strain that can grow on L-arabinose and ferment it to ethanol. The engineered yeasts contain an overexpressing bacterial L-arabinose utilization pathway, which comprises of Bacillus subtilis AraA and Escherichia coli AraB and AraD with concomittant overexpression of L-arabinose-transporting yeast galactose permease.

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Cloning and characterization of the xyl1 gene, encoding an NADH-preferring xylose reductase from Candida parapsilosis, and its functional expression in Candida tropicalis

Article Abstract:

Research describes cloning of Candida parapsilosis mutant strain KFCC-10875xyl1 gene encoding an NADH-preferring xylose reductase and its expression in the yeast Candida tropicalis, which is used in the industrial production of xylitol. The xylose reductase from C. parapsilosis exhibits a dual coenzyme specificity.

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Nuclease activity of Saccharomyces cerevisiae Mre11 functions in targeted nucleotide alteration

Article Abstract:

Research demonstrates the importance of nuclease activity associated with the gene used in targeted nucleotide exchange studies aimed at reengineering yeast genes. Data indicate that Saccharomyces cerevisiae Mre11 gene is endowed with the nuclease activity.

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