A mechanism for production of hydroxyl radicals by the brown-rot fungus Coniophora puteana: Fe(III) reduction by cellobiose dehydrogenase and Fe(II) oxidation at a distance from the hyphae

Article Abstract:

Brown-rot fungus Coniophora puteana was cultivated and processed for the extraction of cellobiose dehydrogenase on which assays for enzyme activities and hydroxyl radical production were performed. It was found that cellobiose dehydrogenase couples oxidation of cellodextrins to conversion of Fe(III) to Fe(II). The results also confirmed that the critical Fe(II)/H2O2 combination is generated at a distance, thus allowing hydroxyl radical production to occur without inflicting damage to the hyphae. This then explains why the fungus does not get killed even with the production of free radicals.

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Enhanced degradation of polyvinyl alcohol by Pycnoporus cinnabarinus after pretreatment with Fenton's reagent

Article Abstract:

A study was conducted to examine effects of chemical and biological treatments to the biodegradation of polyvinyl alcohol (PVA). PVA was preoxidized with Fenton's reagent and inoculated with Pycnoporus cinnabarus. Results indicate that degradation of PVA was enhanced when it was pretreated with FR. No significant increases in its biodegradation level, however, happened when it was exposed to Pycnoporus cinnabarinus.

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The gene pvaB encodes oxidized polyvinyl alcohol hydrolase of Pseudomonas sp. strain VM15C and forms an operon with the polyvinyl alcohol dehydrogenase gene pvaA

Article Abstract:

The products of the pvaA and pvaB genes appear to interact to form an enzyme system for cleaving polyvinyl alcohol. pvaA codes for polyvinyl alcohol (PVA) dehydrogenase and pvaB codes for an oxidized PVA hydrolase.

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