A holistic approach for determining the entomopathogenic potential of Bacillus thuringiensis strains

Article Abstract:

A study was conducted to analyze the crystal protein content of potential of the common bacterium Bacillus thuringiensis subsp. aizawai HD-133 using high-pressure liquid chromatography and a two-step polymerase chain reaction technique. Cultures of the bertha armyworm were put on a semisynthetic diet while purified crystals of B. thuringiensis subsp. aizawai HD-133 were suspended in a buffer solution. Results suggested that plasmid influenced declines in toxicity in HD-133 crystals.

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Binding of Bacillus thuringiensis cryl toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera: Pyralidae): evidence of shared binding sites

Article Abstract:

Bioassays are performed on second-instar larvae of Chilo suppressalis to investigate the activities of Bacillus thuringiensis cry1 endotoxins. Activated toxins and brush border membrane vesicles isolated from C. suppressalis larval midguts are used to analyze binding and competition among cry1Aa, cry1Ac and cry1Ba toxins. Results show that toxins cry1Ac and cry1Ba share a binding site in the midgut membranes. This site is also the low-affinity binding site for toxin cry1Aa.

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Two tsetse fly species, Glossina palpalis gambiensis and Glossina morsitans morsitans, carry genetically distinct populations of the secondary symbiont Sodalis glossinidius

Article Abstract:

Amplified fragment length polymorphism markers are used to investigate genetic diversity among Sodalis glossinidius populations. Strains collected from Glossiba palpalis gambiensis and Glossina morsitans morsitans flies group into separate clusters and this differential structuring might reflect different host-related selection pressures and might be related to different vector competences of Glossina spp.

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