Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis

Article Abstract:

Researchers describe the development of PCR primers specific for Lactobacillus, Pediococcus, Leuconostoc, and Weissella. Lactic acid bacteria make up less than 1% of the intestinal microflora, and are not easily detectable using universal PCR primers.

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Identification of Lactobacillus reuteri genes specifically induced in the mouse gastrointestinal tract

Article Abstract:

Research describes development of a method based on in vivo expression technology for the detection of genes of lactobacilli induced in the murine gut and their functionality. The in vivo expression system is plasmid-based and consists of two reporter genes that identify the induction of specific genes in the gastrointestinal tract.

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A high-molecular-mass surface protein (Lsp) and methionine sulfoxide reductase B (MsrB) contribute to the ecological performance of Lactobacillus reuteri in the murine gut

Article Abstract:

The identification and characterization of a gene encoding a large surface protein (Lsp) and the testing of insertional mutants of the lsp gene and of the three in vivo-induced genes for ecological performance in the reconstituted Lactobacillius-free (RLF) mouse gut is discussed. It is found that homologues of lsp and msrB are present in the genomes of several strains of Lactobacillus and may play an important role in the maintenance of these bacteria in gut ecosystems.

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