A double-selective tissue culture system for isolation of wild-type poliovirus from sewage applied in a long-term environmental surveillance

Article Abstract:

A method using a double-selective culture system was developed to aid environmental surveillance in monitoring the circulation of wild-type poliovirus in sewage systems. The performance of the method was tested by using it to process 1,700 sewage samples carrying both vaccine polioviruses and other enteroviruses. Results indicate that the double-selective culture system method has a poliovirus detection limit of 18 to 50 PFU per one to two liters of sewage and was able to identify 41 wild-type polioviruses.

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Genomic characterization of human and environmental polioviruses isolated in Albania

Article Abstract:

An outbreak of polio in Albania in 1996 was caused by a Sabin-like type 2 poliovirus and an intertypic recombinant Sabin-like type 2/wild type 1 virus. The outbreak affected 138 people in 27 districts and may have been caused by low immunity, poor personal hygiene and improper sewage disposal.

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Assessment of poliovirus eradication in Japan: genomic analysis of polioviruses isolated from river water and sewage in Toyama Prefecture

Article Abstract:

Of 78 poliovirus stains isolated from river water and sewage in Toyama Prefecture, Japan, and analyzed by PCR-restriction fragment length polymorphism, 36 were Sabin vaccine strains and 42 were vaccine variant strains. This indicates that the wild-type virus is not present in the region.

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