A comparative approach to physical and linkage mapping of genes on canine chromosomes using gene-associated simple sequence repeat polymorphisms illustrated by studies of dog chromosome 9

Article Abstract:

A study was conducted to analyze a comparative way to physical and linkage mapping of genes on dog chromosomes. Human or mouse cDNA for genes to be mapped were utilized to isolate cosmid or phage clones from dog genomic libraries. The approach supports gene-associated simple sequence repeat polymorphisms. Results indicated that maps created with the approach can be utilized to integrate anonymous microsatellites with gene maps.

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Individual DNA bands obtained by RAPD analysis of canine genomic DNA often contain multiple DNA sequences

Article Abstract:

A study was conducted to analyze DNA bands of dogs determined by the random amplified polymorphic DNA (RAPD) technique. A DNA template isolated from miniature poodles was utilized to carry out RAPD-polymerase chain reaction. Positive clones were then examined by restriction digestion. Experimental results indicated that RAPD bands amplified from the canine genome support more than one DNA fragment.

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Comparative mapping of the DiGeorge region in the dog and exclusion of linkage to inherited canine conotruncal heart defects

Article Abstract:

Deletions and other rearrangements in the human chromosome 22q11 area lead to DiGeorge syndrome (DGS), with conotruncal heart defects (CTDs) a frequent component. Physical and linkage maps of the canine homolog of the Di-George region were constructed and the question of linkage of CTD to canine genes was examined.

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