Article Abstract:
A chromodomain protein, Swi6, has been found to carry out imprinting functions in fission yeast during meiosis and mitosis. It is involved in imprinting the mat locus. Its transient overexpression changes the epigenetic implant at the mat2/3 region and it stays bound to the mat2/3 interval through the entire cell cycle and seems to be itself a component of the imprint. It converts the expressed state to the silenced one heritably.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
The mating type of Schizosaccaromyces pombe has been found by the mating-type locus (mat1) at chromosome II, and according to the model developed, leading-strand replication brings on a break when the replication fork encounters the imprint lagging-strand replication brings at mat1. Likely swi 1 and swi3 perform imprinting and pausing of DNA replication. Likely they promote imprinting in novel ways, among them terminating replication at RTS1.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
CENP-E, a microtubule-dependent motor protein that extends at least 100 nm from the surface of kinetochores, has been seen to be an essential component of the mitotic checkpoint in vitro. Xenopus egg extracts were studied to look into how CENP-E acts in establishing, maintaining and silencing the mitotic checkpoint. It was surprising that depletion of CENP-E brought failure to establish a checkpoint arrest when challenged with microtubule-depolymerizing agents.
User Contributions:
Comment about this article or add new information about this topic: